Research article rat models of dietinduced obesity and high. Effects of the application of a highfatcarbohydrate diet. The prediabetic weight gain of the zdsd rat is similar to zf and zdf rats. Since rats and mice show a similar relationship, they are considered an appropriate model. Preventing high fat dietinduced obesity and improving insulin sensitivity through neuregulin 4 gene transfer. Greater dietinduced obesity in rats consuming sugar. Pdf modified highsucrose dietinduced abdominally obese and. We developed a rat model of dietinduced obesity that will be a powerful tool for assessment of this issue. The hypercaloric pellet diet cycle promoted an increase in body weight and fat, systolic. Controlled highfat diet induces an obese syndrome in rats.
Control c rats had free access to a standard nonpurified diet containing 51% carbohydrate, 4% fat, and 21% protein. Taconic biosciences maintains an inventory of c57bl6ntac male mice on 60% kcal high fat irradiated diet. Effect of streptozotocin on glucose levels in albino wister rats khaleel nagarchi1, saif ahmed2, anusha sabus3, shaik hussain saheb4. Research article a novel wistar rat model of obesity. Leptin programmed both reduced intake of the obesogenic diets and increased energy expenditure. Rats on a cafeteriastyle caf diet 10 had free access to a semisolid diet containing 33% ground commercial rat chow, 33% full fat sweetened condensed milk nestle, 7% sucrose, and 27% water. In the obese rats, a reduction in nhe3 expression in the proximal tubule may impact on the acidification of endosomes which are responsible for albumin uptake, suggesting a key role for the exchanger in protein endocytosis in obesity. Ro dent mo del for the study of obesityrelated disor ders rev assoc med bras 2012. This allows researchers to study a specific condition, such as obesity, without being effected by the genetic difference. Pdf dietinduced obesity in rats leads to a decrease in sperm. Diet induced obese dio mouse models c57bl6ntac male mice.
A controlled highfat diet induces an obese syndrome in rats. Rats were fed a high fat diet and treated with a low dose of streptozotocin to create models of diet induced obesity or type 2 diabetes. Transcriptomic and metabonomic profiling of op and or rats has been conducted, showing two sets of significantly different phenotypic profiles in response to 16 weeks of high. We hypothesized that excess fuel supply in a rat model of diet induced obesity leads to myocardial cell damage due to increased oxidative stress and apoptosis. This study was designed to investigate the antiobesity activities of ethanolic extract of terminalia paniculata bark tpee on high fat dietinduced obese rats. We investigated the effects of established maternal diet induced obesity on male and female offspring appetite, glucose homeostasis and body composition in rats. Some studies have shown a relationship between obesity and infertility, but until now it remains controversial. The aim of this study was to examine the anti obesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. Highfat diet consumption for 8 weeks affected body weight in longevans but not spraguedawley rats. Protective effects of orlistat on lipid profile, cardiac. Effect of high fatdiet and obesity on gastrointestinal. Established dietinduced obesity in female rats leads to. We examined whether reversal of high fat diet, stimulating weight loss, compared to two treatments previously shown to have beneficial effects, could improve glucose utilization and peripheral neuropathy in animal models of obesity and type 2 diabetes.
Treatingdietinducedobeserats with enalapril or placing them on a normaldiet for weeks, a er weeks of the high fat diet, resulted in diet induced obese rats weighing about the same as control rats at the end of the study. Obesity can be induced in animals by neuroendocrine, dietary or genetic changes. Greater dietinduced obesity in rats consuming sugar solution during the inactive period. The induced obesity resulted from adipocyte enlargement alone, early studies of the development of adipose tissue cellularity in. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. Dietinduced obesity in rats leads to a decrease in. Rodents respond to chronic high fat diet in at least two ways. Thus, the aim of the present study was to investigate the effect of highfat dietinduced obesity on male reproductive parameters.
Modified highsucrose dietinduced abdominally obese and normalweight rats developed high plasma free fatty acid and insulin. Current study comprises usage of a diet induced obesity model by proposing high fat diet on adult female rats and examination of the livers of these obese rats under light microscope. Prevention of dietinduced obesity and impaired glucose. I before the onset of obesity dio rats show an innate decreased sensitivity to the effects of damphetamine on icss threshold and feeding, as compared to dr rats. Rats were calorically restricted on an lf diet to induce 1015% loss, maintained at this reduced weight for 0, 8, or 16 weeks, then allowed to relapse with free access to lf diet. The aim of this study was to examine the antiobesity effects of boiled tuna extract in c57bl6n mice with obesity induced by a highfat diet hfd. The obesity was manifest as a modest but nonetheless significant 10% increase in total body weight. Transcriptomic and metabonomic profiling of obesityprone and. Effect of streptozotocin on glucose levels in albino. The present study used spraguedawley rats that, when fed a highfat diet, exhibit either an obesityprone diop or obesityresistant dior phenotype, to determine whether changes in gut epithelial function and microbiota are diet or obese associated. Young wistar rats 4050 g were fed a hfd 45% energy from fat or a normal diet for 6 month. Accumulating evidence suggests that maternal obesity may increase the risk of metabolic disease in the offspring. Biometric evidence of dietinduced obesity in lewcrl rats article pdf available in comparative medicine 612. Mar 14, 2009 accumulating evidence suggests that maternal obesity may increase the risk of metabolic disease in the offspring.
Yet, many pet rats sadly cant accomplish this trick, because they are overweight. The present study used spraguedawley rats that, when fed a highfat diet, exhibit either an obesity prone diop or obesity resistant dior phenotype, to determine whether changes in gut epithelial function and microbiota are diet or obese associated. Treating diet induced obese rats with enalapril or placing them on a normal diet for 12 weeks, after 12 weeks of the high fat diet, resulted in diet induced obese rats weighing about the same as control rats at the end of the study. We determined the antiobesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of. Nov 24, 2016 obesityprone op rats were maintained on a hf diet for 16 weeks to induce obesity. Young animals have a different metabolism and accumulate less adipose tissue due to the metabolic characteristics appropriate to the age bayol and collaboradors, 2010, it also being advisable that older animalsof approximately 100 days oldshould be subjected to dietinduced obesity rolls, van duijvenvoorde and rowe, 1983. Transcriptomic and metabonomic profiling of op and or rats has been conducted, showing two sets of significantly different phenotypic profiles in response to 16 weeks of high fat diet. Energy expenditure in rats with dietinduced obesity article pdf available in american journal of clinical nutrition 442. A humanized rat model of prediabetes by high fat diet. Jul 10, 2012 greater diet induced obesity in rats consuming sugar solution during the inactive period. Modeling dietinduced obesity with obesityprone rats ncbi.
Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Pdf energy expenditure in rats with dietinduced obesity. Therefore, the dietinduced obese spraguedawley rat may serve as a. Antiobesity effects of boiled tuna extract in mice with. Highfat diet increases pain behaviors in rats with or. Diet induced obesity an overview sciencedirect topics. Current study comprises usage of a dietinduced obesity model by proposing high fat diet on adult female rats and examination of the livers of these obese rats under light microscope. Obesity and adipocyte abnormalities in offspring of rats undernourished during pregnancy. Prenatal influences on leptin sensitivity and susceptibility to dietinduced obesity. C57bl6ntac males are put on special diet at 6 weeks of. Dietinduced obesity in c57bl6j mice causes increased.
Using diet induced obesity to understand a metabolic subtype of osteoarthritis in rats k. Research article a novel wistar rat model of obesityrelated. Jul 15, 2015 however, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf diet induced obesity has not been adequately evaluated so far. Antiobesity and hypolipidemic activity of moringa oleifera. We observed significant differences in transcriptional expression of nearly 80 genes, some of which are known to be involved in lipid metabolism, transport, and. Postprandial colonic response was studied after a high carbohydrate or high fat meal in normal individuals and both meals caused. As mentioned in table 3, it has been observed that rats feed on highfat diet consecutively for 21 days resulted in a marked increase in the level of lipids, characterized by elevated levels of total cholesterol, triglycerides, ldl, vldl, and reduced levels of hdl when compared to normal control, that is, rats receiving the normal feed. Diet induced obese dio mouse models c57bl6ntac male. Rats are known for their ability to squeeze through small spaces and get into everything. Evaluation of antiobesity activities of ethanolic extract of. Dietinduced obesity, adipose inflammation, and metabolic dysfunction correlating with par2 expression are attenuated by par2 antagonism. In conclusion, administration of leptin to pregnant rats during the third trimester of pregnancy and lactation reduced susceptibility to diet induced obesity, glucose intolerance, and insulin resistance in their offspring, especially the females. This dietary induced obesity dio prone rat model of obesity is a outbred, albino, and polygenic model that has been selectively bred to develop obesity associated with impaired glucose tolerance, dysplipidemia, and insulin resistance when fed a highfat, highsucrose, and highcaloric diet.
Emulsifiers in processed foods can lead to an increased risk of obesity, metabolic syndrome. After 4 weeks on either diet, caf rats weighed significantly more than c rats 278. Typically mice, rats, dogs, or nonhuman primates are used in these models. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of dietinduced obesity. Diet induced obesity, adipose inflammation, and metabolic dysfunction correlating with par2 expression are attenuated by par2 antagonism. In the past there has been much debate, and research, over the relationship between type 2 diabetes and obesity.
In stapled rats, segment weight and mucosal weight were lower in the excluded segment, and higher in the transposed segment, compared to all other rats. Obesity and metabolic syndrome are associated with glomerulosclerosis and proteinuria, but the mechanisms are not known. Another author, studying gastric bypass models in spraguedawley rats, induced obesity with food consisting of 48% chow, 8% maize oil and 44% condensed milk for 7 to 12 weeks 48. Rats as a model for obesity research multidisciplinary. Female wistar rats were fed either a standard chow 3% fat, 7% sugar. Dietinduced obesity models diets high in fat have been attributed to a host of diseases, including obesity, metabolic and inflammatory diseases. Rats with free access to an hf diet were fed more energy and became obese relative to rats with free access to a diet containing the same constituents but less fat. The jackson laboratory maintains an inventory of dietinduced obesity dio models, and can place any strain on any fully customized diet so that your mice are ready when you need them. In this study, we used the paradigm of diet induced obesity first described by woods et al. Female wistar rats were fed either a standard chow 3% fat, 7% sugar wtwt or a palatable obesogenic diet 11% fat. Differential effect of honey on selected variables in. Obesity is inflammatory disease, rat study shows medicine.
Data in table 2 demonstrate that feeding rats a high fat diet to induce obesity or subsequent treatment by placing obese rats on a normal diet or treating them with enalapril or menhaden oil enriched diet did not significantly change serum levels of free fatty acids, triglycerides, or cholesterol. Although adverse effects of obesity on female reproduction or oocyte development have been well recognized, its harmfulness to male fertility is still unclear. Greater dietinduced obesity in rats consuming sugar solution. Methods female wistar rats were fed either a standard chow 3% fat, 7% sugar wtwt or a palatable obesogenic diet 11% fat, 43% sugar wtwt for 8 weeks before mating and throughout pregnancy and lactation. Pdf biometric evidence of dietinduced obesity in lew. Rats were fed the same hf purified diet 46% kcal fat. The development of hypertension in a rat model of diet induced obesity is described in the literature. Rat models of dietinduced obesity and high fatlow dose. Propensity to highfat dietinduced obesity in rats is.
In a first experiment, male wistar rats were fed a highfat diet hfd. In conclusion, a sucroserich diet induced obesity, insulin resistance, oxidative stress, and nafld in rats. Effect of streptozotocin on glucose levels in albino wister rats. Dietinduced adipocyte number increase in adult rats. Transcriptomic and metabonomic profiling of obesityprone. Male wistar rats n8group were fed either low fat, western, or high fat diets 10%, 45%, or 60% calories from fat, respectively for acute 17days, short 12 months, intermediate. In this part of the study rats were given a highfat diet. Dietinduced obesity in rats leads to a decrease in sperm. We determined the anti obesity effects of boiled tuna extract 100, 200, or 400 mgkg on the progression of hfd induced obesity for 10 weeks. It is well established that increased availability and utilization of free fatty. In this study, we used the paradigm of dietinduced obesity first described by woods et al. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far.
Physiogenex preclinical cro for the evaluation of your drugs efficacy in metabolic diseases keywords insulin resistance, glucose intolerance liver steatosis, hyperinsulinemic euglycemic clamp, glucoses fluxes, diabetes, pharmacology, in vivo. Introduction over the last decades, obesity has become a global epidemic and an important public health problem in many countries. When compared over 10 wk of free feeding, hf rats weighed 10% more p. Guidelines to increase the effectiveness of the dietinduced obesity model for the model of dietinduced obesity to be e ective, some measures related to the environment where the animal is kept must be observed, especially when rodents without genetic mutations are studied, such as wistar rats. Taconic uses research diets industry standard high fat rodent diet, d12492. Only male zdsd rats develop diabetes hyperglycemia, 500 mgdl and some convert between 15 and 21 weeks of age. Obesity is rapidly becoming a worldwide epidemic that affects children and adults. Rats with blood glucose over 160 mgdl were considered as. Western diet induces apoptosis in the heart of a rat model. The effect of dietinduced obesity on toxicological parameters in. Wistar and sd rats were assigned for 2 experimental groups for. Oxidative stress biomarkers and histology in highfat. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity.
Ii in a diet induced obesity state, dio rats show insensitivity to the damphetamine effect on icss, but show equal sensitivity. Using dietinduced obesity to understand a metabolic subtype. We hypothesized that excess fuel supply in a rat model of dietinduced obesity leads to myocardial cell damage due to increased oxidative stress and apoptosis. Research article rat models of dietinduced obesity and. In dietinduced obese male mice decreases in sperm motility 16,17. The purpose of this study was to determine if there is altered renal lipid metabolism and increased expression of sterol regulatory elementbinding proteins srebps in a model of diet induced obesity. Thus, we tried to understand the effects of obesity on adult female liver.
Selective inbreeding for obesity and diabetes resulted in a strain that exhibits these characteristics without the lepr fa mutation. The induced obesity resulted from adipocyte enlargement alone, early studies of the development of adipose tissue cellularity in rats and mice using cellcounting tech. Dietinduced obesity in c57bl6j mice causes increased renal. The rats observed were those in the authors own rattery. Diet induced obese rats weighed significantly more than control rats after 24 weeks on the high fat diet. However, transgenic rats do exist for research purposes. Wistar and sd rats were assigned for 2 experimental groups for 17 weeks. Type i dm was induced in 20 rats by a double ip injection of stz sigma as 30 mg kg for two consecutive days with a total of 60 mg kg rat. About 15% of the rats refused to run on a treadmill and were excluded from the study at the end of the preliminary stage. Thus, the aim of the present study was to investigate the effect of highfat diet induced obesity on male reproductive parameters. Mar 11, 2011 obesity is rapidly becoming a worldwide epidemic that affects children and adults. This study characterizes the highfat diet hfdfed rats as a model for prediabetes or impaired glucose tolerance showing clinical presentation and pathophysiology of natural history of prediabetes in human.
Modeling dietinduced obesity with obesityprone rats. Using dietinduced obesity to understand a metabolic. It is assumed that honey would not reduce the level of hyperglycaemia induced by fructose, even if it does in the rats with alloxaninduced dm. In the past there has been much debate, and research, over the relationship between type 2. Guidelines to increase the effectiveness of the diet induced obesity model for the model of diet induced obesity to be e ective, some measures related to the environment where the animal is kept must be observed, especially when rodents without genetic mutations are studied, such as wistar rats. A new rat model zdsd of type 2 diabetes independent of. Using dietinduced obesity to understand a metabolic subtype of osteoarthritis in rats k. Obesity is a complex metabolic disease that is a serious detriment to both children and adult health, which induces a variety of diseases, such as cardiovascular disease, type ii diabetes, hypertension and cancer.
Effect of high fatdiet and obesity on gastrointestinal motility. It is intended to mimic the most common cause of obesity in humans. Preventing high fat dietinduced obesity and improving. The model of diet induced obesity in rodents can be considered effective when the objective is the study of the physiopathology of metabolic and vascular complications associated with obesity. We investigated the effects of established maternal dietinduced obesity on male and female offspring appetite, glucose homeostasis and body composition in rats. C57bl6ntac males are put on special diet at 6 weeks of age and. May 01, 2017 young animals have a different metabolism and accumulate less adipose tissue due to the metabolic characteristics appropriate to the age bayol and collaboradors, 2010, it also being advisable that older animalsof approximately 100 days oldshould be subjected to diet induced obesity rolls, van duijvenvoorde and rowe, 1983.
701 100 1486 1248 38 1296 366 214 1511 1501 1220 457 1600 542 1178 1180 170 1612 460 132 1566 716 1108 417 629 790 708 1402 1097 828 287 707